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Chapter: Pharmaceutical Biotechnology: Fundamentals and Applications - Growth Hormones

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hGH Receptor and Binding Proteins

The hGH receptor is a member of the hematopoietic cytokine receptor family.

hGH Receptor and Binding Proteins

 

The hGH receptor is a member of the hematopoietic cytokine receptor family. It has an extracellular domain consisting of 246 amino acids, a single 24 amino acid transmembrane domain, and a 350 amino acid intracellular domain (Fisker, 2006). The extra-cellular domain has at least six potential N-glycosyla-tion sites and is usually extensively glycosylated. hGH receptors are found in most tissues in humans. However, the greatest concentration of receptors in humans and other mammals occurs in the liver (Mertani et al., 1995).


 


As much as 40% to 45% of monomeric hGH circulating in plasma is bound to one of two binding proteins (GHBP) (Fig. 4) (Fisker, 2006). Binding proteins decrease the clearance of hGH from the circulation (Baumann, 1991) and may also serve to dampen the biological effects of hGH by competingwith cell receptors for circulating free hGH. The major form of GHBP in humans is a high affinity (Ka ¼ 10–9 to 10–8 M), low capacity form which preferentially binds the 22 kDa form of hGH (Herington et al. 1986; Baumann, 1991). Another low affinity (Ka ¼ 10–5 M), high capacity GHBP is also present which binds the 20 kDa form with equal or slightly greater affinity than the 22 kDa form. In humans, the high affinity GHBP is identical to the extracellular domain of the hGH receptor and arises by proteolytic cleavage of hGH receptors by a process called ecto-domain shedding. Since the high affinity binding protein is derived from hGH receptors, circulating levels of GHBP generally reflect hGH receptor status in many tissues (Hansen, 2002; Fisker, 2006).

 

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