Culture
Methods
Introduction
Culture methods are very crucial in a microbiology laboratory.
Various culture methods are carried out to:
a)
Isolate bacteria in pure culture and identify the same by
performing various tests.
b)
Demonstrate biochemical, antigenic, and other phenotypic and
genomic properties of the isolated colonies.
c)
Demonstrate susceptibility of the isolated bacteria to antibiotics,
bacteriophages, bacteriocins, etc.
d)
Prepare antigens for various uses.
e)
Maintain stock culture.
Methods of Culture
Various methods are used for culturing of bacteria. These include (a) streak culture, (b) lawn culture, (c)
pour-plate culture, (d ) stroke
culture, (e) stab culture, and ( f ) liquid culture.
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