Home | | Genetics and Molecular Biology | Biology of 5S RNA Synthesis in Xenopus

Chapter: Genetics and Molecular Biology: Xenopus 5S RNA Synthesis

Biology of 5S RNA Synthesis in Xenopus

Oocytes develop in the ovaries of female frogs in a development process that takes from three months to several years.

Biology of 5S RNA Synthesis in Xenopus

Oocytes develop in the ovaries of female frogs in a development process that takes from three months to several years. Eventually the oocytes mature as eggs ready to be fertilized. These huge cells contain large amounts of nutrients and protein synthesis machinery so that later, the fertilized egg can undergo many cell divisions without significant intake of nutrients and without additional ribosome synthesis.


Curiously, during oogenesis components of the ribosomes are not synthesized in parallel. In the ovaries the 5S RNA components of ribosomes are synthesized first, for about two months. As they are synthesized, they are stored away in two types of RNA-protein particles.

Figure 15.1 Two rounds of initia-tion from an origin can yield four copies of genes immediately sur-rounding that origin.

These are known as 7S and 42S particles from their rates of sedimenta-tion in centrifugation. Even though 5S RNA synthesis proceeds for a long time, the enormous number of RNA molecules needed in an egg necessitates that multiple genes encode the RNA. The Xenopus genome contains about 100,000 copies of the 5S gene. A special RNA polymerase transcribes these genes as well as genes coding for tRNAs. This is RNA polymerase III. Although it is different from the two other cellular RNA polymerases, it shares a number of subunits in common with them.


After synthesis of the 5S RNA, synthesis of the 18S, 28S, and 5.8S ribosomal RNAs and the ribosomal proteins begins. We might expect the frog genome also to contain around 100,000 copies of the genes coding for 18S, 28S and 5.8S ribosomal RNAs. It doesn’t, however. Apparently the load of carrying large numbers of these much bigger genes would be too great. Instead, Xenopus generates the required number of DNA templates for the other ribosomal RNAs by selective replication of the 450 ribosomal RNA genes before the ribosomal RNA is to be synthesized.

Once, a key to success in developmental biology was finding an organism or cell-type devoted to the activity you wished to study. In such cells the specialization in activity reduces the level of other and inter-fering activities. This simplifies the experimenter’s measurements. Also, the purification for biochemical study of the components involved is simpler since they are present in sizeable concentrations in these cells. For this reason Xenopus permitted careful examination of several im-portant developmental biology questions before the appearance of genetic engineering techniques.

The strategy of selective gene amplification used by Xenopus on its ribosomal RNA genes is not unique. Other organisms also utilize it for genes whose products are needed in high level. In terminally differenti-ated tissues where no further cell division occurs, the gene amplification can occur right on the chromosome by the selective activity of a replication origin (Fig. 15.1). Since an egg cell, however, must later divide, the mechanism of gene amplification must not irreparably damage the genome. In Xenopus a copy of the rRNA gene is replicated

Figure 15.2 Selective replication of rRNA genes yields as many as 4,000,000gene copies per cell.


free of the chromosome to generate high numbers of templates neces - sary for the synthesis of the RNA (Fig. 15.2). As the last components of a ribosome are synthesized, mature ribosomes form. Some begin trans-lating maternal mRNA while others remain inactive until fertilization triggers a more vigorous rate of protein synthesis.


Why the genes coding for 5S RNA cannot be amplified along with the 18S, 28S, and 5.8S rRNA genes is not apparent. Xenopus, and most other organisms, have gone to considerable trouble to separate the synthesis of 5S and the other ribosomal RNAs. Even the RNA polymerases used are different: 5S uses RNA polymerase III whereas 18S, 28S, and 5.8S use polymerase I.

As the oocyte matures, ribosome synthesis and most other activity ceases until fertilization activates protein synthesis. Then, after the developing embryo has reached the 4,000 cell stage, 5S ribosomal RNA synthesis begins again. A small fraction of the RNA synthesized at this time is the RNA species which was synthesized in the oocyte. Most, however, possesses a slightly different sequence. It is called somatic 5S RNA. The oocyte 5S RNA synthesis soon ceases altogether, leaving just the somatic 5S RNA synthesis. The haploid genome of Xenopus contains about 450 copies of the somatic variety of 5S gene in contrast to about 100,000 copies of the variety expressed only in oocytes.

Study of 5S RNA synthesis is attractive because the 5S genes show developmental regulation in that the oocyte 5S genes are expressed only in the developing oocyte whereas the somatic 5S genes are expressed both in the oocyte and in the developing embryo. In addition to the simple timing pattern of 5S RNAs, the product of the synthesis is simple. The 5S RNA is not processed by capping, polyadenylation, or splicing, nor is there a tissue specificity in the expression. Hence this seems like an ideal material for study of developmental questions.

Study Material, Lecturing Notes, Assignment, Reference, Wiki description explanation, brief detail
Genetics and Molecular Biology: Xenopus 5S RNA Synthesis : Biology of 5S RNA Synthesis in Xenopus |

Privacy Policy, Terms and Conditions, DMCA Policy and Compliant

Copyright © 2018-2024 BrainKart.com; All Rights Reserved. Developed by Therithal info, Chennai.