Virus Isolation and Animal Susceptibility - Adenovirus

Virus Isolation and Animal Susceptibility

Human adenoviruses are highly host specific; hence laboratory animals are not susceptible to infection by the virus.

◗ Cell culture

Human adenoviruses can be grown only in tissue cultures of human origin. They grow well on monolayer of HeLa, Hep-2, KB, and human embryo kidney cells. Cytopathic changes are observed after 1–4 weeks of inoculation and consist of cell rounding and aggregation into grape-like clusters. Infected cells swell, become ballooned, and show intranuclear inclu-sions in stained preparations.