Human adenoviruses are highly host specific; hence laboratory animals are not susceptible to infection by the virus.
Human adenoviruses can be grown only in tissue cultures of human origin. They grow well on monolayer of HeLa, Hep-2, KB, and human embryo kidney cells. Cytopathic changes are observed after 1–4 weeks of inoculation and consist of cell rounding and aggregation into grape-like clusters. Infected cells swell, become ballooned, and show intranuclear inclu-sions in stained preparations.