Milestones
in Plant Tissue Culture
Haberlandt (1902)
cultured
plant cells in artificial condition called in vitro (inside glass) in culture
medium (Knop’s salt solution) containing glucose and peptone and developed
callus (unorganized growth of cells and tissue) and proposed the concept
Totipotency, it means the development of whole plant from isolated cells or
tissue in in vitro condition.
P.R.White (1934)
developed
root cultures, used Knop’s solution along with three vitamins like pyridoxine,
thiamine and nicotinic acid
F.C. Steward (1948)
used
coconut water in plant tissue culture work and obtained cell proliferation from
carrot explants (Cellular totipotency ).
Morel and Martin (1952, 1955)
developed
virus-free Dahlia and potato plants using shoot meristem culture.
Murashige and Skoog (1962)
formulated
tissue culture medium, a land mark in plant tissue culture and it is the most
frequently used medium for all kinds of tissue culture work.
Kanta et al. (1962)
produced
test-tube fertilization in flowering plants.
Yamada et al. (1963)
produced
calli and free cells in tissue culture of Tradescantia reflexa.
Guha and Maheshwari (1964)
developed
in vitro production of haploid embryos from anthers of Datura.
Vasil and Hildbrandt (1965)
achieved
differentiation of tobacco plants from single, isolated cells in micro
propagation.
Takebe et al. (1971)
regenerated
tobacco plants from isolated mesophyll protoplasts.
Carlson
and
co-workers obtained protoplast fusion between Nicotiana glauca and Nicotiana
longsdorffii and developed first interspecific somatic hybrid in 1971.
Melchers and co-workers in 1978
developed
intergenic hybrid between potato and tomato called pomato.
Chilton (1983)
produced
transformed tobacco plants from single cell transformation and gene insertion.
Horsh et al. (1984)
developed
transgenic tobacco by Agrobacterium mediated gene transfer.
Knop’s solution: Nutrient solution used in growth
experiments of plants which contains:
Calcium
nitrate 3.0 g
Potassium
nitrate 1.0 g
Sucrose
50.0 g (optimal)
Magnesium
sulfate 1.0 g
Dibasic
Potassium phosphate 1.0 g
Deionized
water 1000.0 ml
Related Topics
Privacy Policy, Terms and Conditions, DMCA Policy and Compliant
Copyright © 2018-2024 BrainKart.com; All Rights Reserved. Developed by Therithal info, Chennai.