A variety of special stains are employed in the surgical pathology laboratory. Most of these are performed by specially trained laboratory per-sonnel, and a detailed description of all of these stains is beyond the scope of this manual. The surgical pathologist and pathologist’s assistant, however, should be able to perform a few basic stains: hematoxylin and eosin (H&E), oil red O (ORO), periodic acid-Schiff (PAS), Gram Weigert’s (GW), Ziehl-Neelsen, and Papanicolaou (PAP) stains.
While the details of each stain vary from labo-ratory to laboratory, examples of ‘‘cookbook-style’’ instructions for the performance of each of these six stains on frozen tissue sections follow.
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