Fluorometry - Analysis of biomolecules

Fluorometry

Flurometry is based on the phenomenon whereby a molecule after absorbing radiation emits radiation of longer wavelength which is known as fluorescence. It is a short lived phenomenon about 10-7 seconds. Fluorometry is an analytical tool which can be used for determination of very small concentration of substances. As that of UV Visible spectroscopy Beer Lamberts law is also applied to the Fluorometry. Some of the applications of spectrofluorometry are;

·        Qualitative analysis

·        Quantitative analysis of vitamins, cortisol, serotonin, dopamine etc

·        Assay of oraganophosphorus pesticides, carcinogens, drugs and even some metal ions.

Flame spectroscopy

It is the absorption or emission of specific wavelength by exited atoms which is taken by flame. There are two types in flamespectroscope viz; Emission Flame photometry and Atomic Absorption Spectrophotometry. In Emission Flame photometry, the characteristic emission spectrum of the element is produced when the excited atoms return to their ground state. Whereas, in Atomic Absorption Spectrophotometer it measures the absorption of a beam of monochromatic light by atoms in a flame.

Applications

·        Assay of macro and microelements in blood, plasma, urine, saliva, CSF, milk, tissues, soil samples and plants.

·        Flame photometry is especially used in estimation of alkali, alkaline earth and rare earth materials.

·        They can be used to estimate gold, silver, iron, lead, copper, zinc and other elements in the biological samples and as well as in animal feed and plant materials