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Chapter: Medicine Study Notes : Gastro-Intestinal

Assessment - Liver Disease

NB: This section combines notes from the Gut run, and from Microbiology and Chemical Pathology

Liver Disease

 

·        NB: This section combines notes from the Gut run, and from Microbiology and Chemical Pathology

 

Assessment

 

Presentation

 

·        Found coincidentally when clinically well (e.g. medicals)

·        Family screening e.g. abnormal irons, HBV 

·        Non-specific illness and found to have abnormal LFT: ?alcohol, medicines, viral, jaundice, liver/bilary disease

·        Is it acute, chronic, acute on chronic, or a failing chronic

 

Aetiology

 

·        Acute hepatitis:

o  Viral: A, B, C, D, others

o  Toxic:

§  Drugs

§  Poisoning (eg alcohol)

·        Chronic hepatitis:

o  Viral: B, C, B + D, CMV

o  Toxic:

§  Drugs

§  Chronic Alcohol

o  Autoimmune

o  Metabolic:

§  Alpha-1-antitrypsin

o   Wilson‟s disease

o   Haemochromatosis

·        Fatty liver: Obesity, NIDDM, Drugs, alcohol

 

History

 

·        Surgery: e.g. cholecystectomy

·        Heart disease: congestive heart failure

·        Pregnancy

·        HIV positive, sexual orientation

·        Contact with hepatitis: family, sexual

·        Travel: malaria, parasites

·        Hepatitis infection risk: Tattoos, body piercing, health care worker, transfusion, IVDU 

·        Alcohol ® acute alcoholic hepatitis

·        Ethnic group

·        Medications – ALWAYS ASK ABOUT DRUGS 

·        Family History: e.g. anaemias, Gilbert‟s syndrome, haemochromatosis, gallstones, alcoholism, autoimmune disease, Wilson‟s disease (copper) 

·        Symptoms: itch, urine and stool colour

 

Liver Function Tests (LFTs)

 

·        Investigations:

o   Bloods:

§  Bilirubin

§  LFT: AST, ALT, ALP, GGT

§  Total protein, albumin

§  Tests can be widely variable for the same condition

§  Other: Coagulation studies, ammonia 

§  Special tests: a1 antitrypsin, afeta protein, hepatitis markers, specific autoantibodies, Igs, caeruloplasmin 

§  Any liver disorder may ® ­ferritin (also an acute phase protein)

 

o   Also test for other causes of liver disease: HBV, HIV, iron studies (ferritin), immune liver disease (­Anti-nuclear Antibodies – ANA) 

o   Imaging: ultrasound +/- CT, MRCP, ERCP. Ultrasound and CT have high false negatives for bilary

o   Percutaneous transhepatic cholangiogram – PCT

o   Liver biopsy the gold standard

·        Aim to decide if liver disease is present, is progressing or is severe 

·        Aiming to answer three questions:

·        Is there liver cell death – inflammation and hepatonecrosis?  Check:

o   Bilirubin: in acute hepatitis will be 50:50 direct and indirect 

o   Raised aminotransferases predominate. If AST > ALT think severe cirrhosis, liver malignancy, alcohol. In normal hepatitis ALT > AST

o   Common liver causes:

 

§  Non-alcoholic steatohepatitis (fatty liver): probably the most common cause of mildly elevated LFTs, especially if obese, Type 2 diabetes and hyperlipideamia

§  Acute/chronic viral hepatitis

§  Genetic haemochromatosis

§  Autoimmune hepatitis 

§  Less commonly: a1 antitrypsin deficiency and Wilson‟s disease

o   Causes of abnormal LFTs other than Liver disease:

§  Diseases of other organs affecting liver, e.g. RA

§  Medicines, alcohol, tonics, remedies, poisons 

§  Congestive heart failure ® hepatic congestion

o   AST: also produced by:

 

§  Muscle:  If normal ALT and ­AST then do a CK for muscle breakdown

§  Blood: Haemolysis


·        Is there cholestasis (=impaired bile flow)? Liver can remove 5 * normal bilirubin from circulation (i.e. large functional reserve). Cholestasis usually refers to obstruction within the liver. „Obstructive Jaundice‟ Þ major ducts. Bile salts are 90% reabsorbed in the terminal ileum. They emulsify fats. Bile also contains cholesterol, phospholipids and bilirubin (reabsorbed ® urobilinogen ® urine)

 

o  If left or right hepatic duct blocked, other side of liver will be sufficient to keep bilirubin normal

 

o  GGT released in inflammation: usually in parallel with ALP in obstruction. If ­GGT and ­ALP then ALP is from the bilary tree

 

o  Raised ALP and GGT predominate in cholestatic diseases


o  Common:

§  Biliary obstruction: gallstones

§  Drug hepatotoxicity

§  Neoplasms (eg head of pancreas)

o  Less Common:

§  Primary biliary cirrhosis

§  Primary sclerosing cholangitis

§  Sarcoidosis

§  Autoimmune cholangiopathy

 

o  Other causes of ­ALP:

 

§  ALP from bone and cholangiocytes (bilary epithelium). Excreted in urine, but saturated kinetics ® ­serum level

§  Physiological:

·        Bone: Growth and fractures.  High in puberty

·        Pregnancy (placental)

·        Benign ­ with age

§  Bone disease: Paget‟s, malignancy, renal failure, hyperparathyroidism, Rickets

§  GI tract can also produce ALP, eg Crohn‟s

§  Miscellaneous: hyperthyroidism, familial benign, transient of infancy

 

o  GGT ­ benignly with age and obesity

 

·        Is liver function normal? Are detoxification, synthesis, and glucose management working? Has a large functional reserve. Check:

 

o  Bilirubin

o  Albumin.  See below 

o  Prothrombin time (INR): factors 2, 5, 7, 9, 10. Give parental Vitamin K to differentiate between malabsorption or poor liver function

 

·        Tests in cirrhosis

o  Liver function tests very variable:

 

§  Quiescent phase: normal of minor ­ in LFT

§  Active Phase: ­ in ALT and AST when necrosis is dominant

 

o  Causes: idiopathic, alcohol, chronic active hepatitis, primary biliary cirrhosis, haemochromatosis, Wilson‟s disease, a1 antitrypsin deficiency

·        Other examples of Liver Function tests:

 

o  ­­ALT and ­­AST: viral hepatitis, Paracetamol OD 

o  ­ALP and ­bilirubin in a 12 year old with vomiting: Gilbert‟s syndrome (­bilirubin when fasting), ALP normally raised at this age 

o  ­Bilirubin, ­ALP, ¯Albumin + neuro signs ® ?Wilson‟s disease (very rare)

o  ­Bilirubin, ­ALP, ¯Albumin + abnormal electrophoresis ® ? ¯a1 antitrypsin

 

Total Protein

 

·        Normal Ranges:

 Total Protein 60 – 80

Albumin 34 – 46 

Globulin Gap ~ 20  

 

·        Examples:

 

o  55 year old man, ¯albumin, normal protein Þ ­Ig (common in cirrhosis)

 

o  14 year old, ­cholesterol, ¯protein, ¯albumin Þ nephrotic syndrome (relevance of high cholesterol not understood)


o   58 year old man with diabetes, protein 94, albumin 56 Þ dehydration (don‟t get albumin > 50 without dehydration)

 

o   40year old post-op, protein 26, albumin 11 Þ dilution.  Took blood downstream of iv line

 

o   46 year old, enlarged nodes, protein 50, albumin 33 Þ ¯ globulin gap Þ ? immunocompromised/lymphoma

 

o   60 year old, pneumonia, protein 70, albumin 22 Þ acute phase + maybe ­Ig


o   50 year old, recurrent abdominal pain, protein 55, albumin 27 Þ pancreatitis ® malabsorption

 

o   38 year old, SOB, rash, protein 86, albumin 34 Þ sarcoidosis or SLE

·        Differentials:

o   Hypoproteinaemia:

§  Haemodilution: poor iv therapy, drip arm, SIADH, pregnancy

 

§  ¯Albumin: ¯synthesis (liver disease, malabsorption, malnutrition), losses (renal, gut, skin), non-specific (eg illness)

 

§  ¯Ig: primary or secondary immunodeficiencies. Only IgG deficiency is enough to show up as low protein or on electrophoresis

 

o   Hyperproteinaemia:

§  Haemoconcentration: dehydration, haemostasis

 

§  ­Ig:

·        Monoclonal: myeloma, lymphoma, macroglobulin, MGUS

·        Polyclonal: liver disease, infection, autoimmune, sarcoidosis

·        Oligoclonal

 

Aside: Electrophoresis

 

·        Bands: albumin, a1 antitrypsin, haptaglobins (a2 band), transferrin, complement, Igs

·        Two indications:

o   a1 antitrypsin deficiency

o   Monoclonal antibody band

·        Monoclonal Gammaglobulinaemia of Uncertain Significance (MGUS) 

o   Benign, but potential for malignant transformation (eg to Myeloma): 5% at 5 years, 25% at 15 years 

o   Þ need to follow up over time

o   See Aside: Conditions associated with Monoclonal proteins

 

Causes of Jaundice

 

·        For neonatal jaundice

·        Unconjugated hyperbilirubinaemia

o   Overproduction: Intravascular or extrahepatic haemolysis 

o   ¯ Hepatocellular uptake: drugs, sepsis, starvation 

o   ¯ Hepatocellular conjugation: Gilbert‟s syndrome, neonatal jaundice, drugs, diffuse hepatic disease

 

·        Conjugated bilirubinaema (cholestatic jaundice)

o   Impaired hepatocellular secretion: various syndromes, duct stricture, biliary cirrhosis, steroids 

o   Hepatocellular cholestasis – impaired secretion plus liver injury as well: viral infection, drugs, alcohol

o   Extrahepatic obstruction: stones, carcinoma, strictures and congenital atresia

 

Pathology

 

·        Normal histology:

o   Chords of hepatocytes between sinuses running from portal tract to central vein

o   Zone 3 (perivenular) more sensitive (eg to drugs) than zone 1 (periportal)

·        Acute Hepatitis:

o   =Inflammation of the liver: no cause implied

o   Macroscopic appearance: mildly enlarged, tender liver.  Flu like symptoms.  Jaundice, itching

o   Microscopic appearance:

§  Diffuse liver cell injury with lobular disarray: loss of normal radial array 

§  Focal necrosis of hepatocytes with hepatocyte regeneration (mitotic figures, variation in cell size) 

§  Portal inflammation: lymphocytes & macrophages.  Eosinophils with drugs

§  Bile stasis: variable (often absent), due to disruption of canaliculi, greenish

§  NO fibrosis

·        Chronic hepatitis:

o  If longer than 6 months then chronic – otherwise acute (unless chronic signs – e.g. spider naevi)

 

o  Chronic active and chronic persistent hepatitis reflect different disease activity but caused by the same agents

 

o  Chronic persistent hepatitis: Benign and self-limiting, following acute hepatitis and lasting several years. Inflammation limited to portal triad (lymphocytes, macrophages and plasma cells). Architecture preserved, no fibrosis, no hepatocyte necrosis

 

o  Chronic active hepatitis:

§  Progressive hepatic necrosis and fibrosis, potentially leading to cirrhosis

§  Clinical course variable.  5 year survival 25 – 50%

§  Marked portal inflammation extending into lobules 

§  Piecemeal necrosis: „nibbling away‟ at hepatocytes around portal track by lymphocytes ® necrosis

§  Bridging necrosis: creating portal-central or portal-portal tracts

§  Hepatitis B: ground glass hepatocytes – large, uniform cytoplasm filled with viral protein

§  Hepatitis C: fatty degeneration, bile duct lesions and lymphoid follicles

·        Fulminant Hepatitis:

o  Very rare.  Onset to death in 2 – 3 weeks (massive necrosis) to 3 months (submassive failure) 

o  Causes: Viral (60%), drugs/chemicals (30%, eg paracetamol poisoning), numerous other minor causes

o  Macroscopic appearance: all/most of liver destroyed.  Red, limp, wrinkled capsule.  Mushy red 

o  Microscopic appearance: Zonal (2,3) or complete necrosis, liquefaction of hepatocytes, little inflammation

 

Management of Acute Hepatitis

 

·        Avoid strenuous physical activity while jaundiced, increase slowly afterwards

·        Best rest only if unwell

·        No corticosteroids

·        Perhaps reduce fat, abstinence from alcohol

·        Care with personal hygiene

·        Follow liver tests until normal

 

Progression of Chronic Hepatitis

 

·         Acute hepatitis (inflammation/cell death) ® regeneration ® resolution or chronic hepatitis, which may ® fibrosis (the necessary step for progression to liver failure) ® disordered architecture ® cirrhosis

·        Assessing Liver Biopsy:

 

o  Is there inflammation around the portal tract? „Limiting plate‟ surrounds portal tract. Monocytes migrate through this and cause piecemeal or interface hepatitis. If it extends to another portal tract then called bridging necrosis 

o  Is there inflammation/necrosis out in the lobule Þ intralobular or focal necrosis

o  Are there inflammatory cells in the portal tract Þ portal inflammation

o  How much scar tissue/fibrosis is there?

o  Knodell index scores each of these to generate a Hepatitis Activity Index (HAI).  Score out of 22

 

·        Child-Pugh Classification of liver function/failure: sum of scores for encephalopathy, ascites, bilirubin, albumin, INR, nutrition


 


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