Calibrations,
Standardizations, and Blank Corrections
We introduced a relationship between
the measured signal,Smeas, and the absolute amount of analyte
Smeas = knA +
Sreag ……… 5.1
or the relative amount of analyte in a sample
Smeas = kCA + Sreag ……… 5.2
where nA is the moles
of analyte, CA is the analyte’s concentration, k is
the method’s sensitivity, and Sreag is the contribution to Smeas from constant errors introduced by the reagents used in the
analysis. To obtain an accurate value for nA or CA it is necessary to avoid determinate errors affecting Smeas, k, and
Sreag. This is accomplished by a combination of calibrations, standardizations, and reagent blanks.
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